CD74 (MHC II Li Chain) (clone EBS-CD-041), anti-human

Source:
A BALB/c mouse was immunized with B lymphoblastoid cell line HFB1.
Fusion partner: NS-1.



Specifications:
The CD74 cluster, established during the IVth and Vth Leukocyte Typing Workshops, comprises four species of proteins (MW 41/35/33 kDa), all coded by a single gene, consisting of nine exons. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. In tissue sections anti-CD74 show a binding pattern very similar to that of anti-HLA-DR. It binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. EBS-CD-041 epitope is localized in the extracellular domain of CD74.

Species reactivity:
Positive: human.

Applications:
Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Western blot: +

Positive control: Daudi cells, Raji cells, tonsil, lymph node.

References:
- Epstein A.L. et al. J. Immunol.133: 1028-1036 (1984).
- Marder, R.J. et al. Lab. Invest.52: 497-504 (1985).
- Lazova R. et al. Cancer79: 2115-2124 (1997).
- Pich, A. et al. Eur J Basic Appl Histochem.35(1): 81-9 (1991)