CD74 (MHC II Li Chain) (clone LN-2), anti-human, mouse, baboon

Source:
A BALB/c mouse was immunized with cell extracts from diffuse histiocytic lymphoma SU-DHL-4.
Fusion partner: NS-1.



Specifications:
LN-2 is directed against the CD74 cluster, established during the IVth Leukocyte Typing Workshops. CD74 comprises four species of proteins (MW 41/35/33 kDa), all coded by a single gene, consisting of nine exons. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. In tissue sections anti CD74 show a binding pattern very similar to that of anti-HLA-DR. It binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides.

Species reactivity:
Positive: baboon, human, mouse. Negative: rat.

Applications:
LN-2 is used for identification of germinal center derived B-cell lymphomas, leukemias, diagnostic Reed-Sternberg cells and Hodgkin’s cells. Anti-VD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histocytoma (+).
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: Daudi or Raji cells. Tonsil or lymp node.

References:
- Epstein A.L. et al. J. Immunol.133: 1028-1036 (1984).
- Marder, R.J. et al. Lab. Invest.52: 497-504 (1985).
- Lazova R. et al. Cancer79: 2115-2124 (1997).
- Pich, A. et al. Eur J Basic Appl Histochem.35(1): 81-9 (1991).