CD80 polyclonal, anti-rat

Quantity: 100 µg

Background:
Cluster of Differentiation 80(also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28(for autoregulation and intercellular association) and CTLA-4(for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. Reeves et al.(1997) stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

Description:
Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD80 (CD80) detection. Tested with WB in Rat.

Synonyms:
Activation B7 1 antigen antibody, Activation B7-1 antigen antibody, Activation B71 antigen antibody, B lymphocyte activation antigen B7 antibody, B7 antibody, B7-1 antibody, B7-1 antigen antibody, BB1 antibody, CD 80 antibody, CD28 antigen ligand 1 antibody, CD28 antigen ligand 1 B7 1 antigen antibody, CD28 antigen ligand 1 B71 antigen antibody, CD28LG antibody, CD28LG1 antibody, CD80 antibody, CD80 antigen antibody, CD80 antigen precursor antibody, CD80 molecule antibody, CD80_HUMAN antibody, Costimulatory factor CD80 antibody, CTLA 4 counter receptor B7.1 antibody, CTLA-4 counter-receptor B7.1 antibody, LAB 7 antibody, LAB7 antibody, T lymphocyte activation antigen CD80 antibody, T-lymphocyte activation antigen CD80 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of rat CD80(227-245aa DAHVSQNFTWEKPPEDPPD), different from the related mouse sequence by two amino acids.A synthetic peptide corresponding to a sequence at the C-terminus of rat CD80(227-245aa DAHVSQNFTWEKPPEDPPD), different from the related mouse sequence by two amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: rat

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.