CD84 (GR6) (clone 153-4D9), anti-human

Source:
A BALB/c mouse was immunized with MLR stimulated lymphocytes.
Fusion partner: NS-1.



Specifications:
153-4D9 reacts with human CD84 (Mw 74 kDa). CD84 is expressed on mature B-cells and on B-cell line but not on plasma cell lines. Immunohistochemistry demonstrated that is strongly stains tissue macrophages. It is also expressed on platelets and at low levels on blood T-cells. It is a highly N-glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation but cellular expression does not significantly increase after activation. 153-4D9 was clustered at the Vth WLDA.

Species reactivity:
Positive: human.

Applications:
153-4D9 can be used for frozen sections, immunofluorescence, flow cytometry and immunoblotting.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Western blot: +

Positive control: Raji, HL60, THP-1, HMC-1, HEL and NAMALWA cells. Human platelets, macrophages and tonsil.

References:
- 1.Schlossman SF et al. (eds) Leukocyte Typing V. p.699-700, Oxford University Press, Oxford, (1995).
- De la Fuente, M, et al, Blood 90(6): 2398-2405 (1997).