CD8a (clone EBS-CD-009), anti-human

Source:
A BALB/c mouse was immunized with human PBL.
Fusion partner: NS-1.



Specifications:
EBS-CD-009 recognizes a protein of 32 kDa, identified as CD8a. The CD8 molecule consists of α and β-chain, which are disulphide-linked into heterodimers or homodimers. CD8 is expressed on a T-cell subset (cytotoxic/suppressor T-cells), thymocytes and NK-cells. The majority of CD8+ T-cells express CD8 as heterodimer. Some subpopulation of SD8+ T-cells as well as NK-cells may express homodimer. CD8 functions as a co-receptor in concert with TCR for binding the MHC class I/peptide complex. HIV-2 envelope glycoprotein binds CD8 α-chain but not β-chain.

Species reactivity:
Positive: human.

Applications:
Characterization of leukemia and lymphoma.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: ―

Positive control: HuT78 or hPBL. Tonsil.

References:
- Knapp W. et. al. Leukocyte Typing IV, p342-343, Oxford University Press, (1989)
- Parnes JR, Semin Immunol6: 221-229 (1994).
- Delon J. et al. Immunity9(4): 467-73 (1998).
- Akimoto H, et al. Immunology95(2): 214-218 (1998).
- Leahy DJ. Transplantation45(4): 677-682 (1998).