CD8a (clone RIV11), anti-human

Source:
A BALB/c mouse was immunized with human PBL.
Fusion partner: P3-X63-Ag8.653.



Specifications:
RIV11 recognizes a protein of 32 kDa, identified as CD8a. The CD8 molecule consists of α and β chains, which are disulphide-linked into heterodimers or homodimers. CD8 is expressed on a T-cell subset (cytotoxic/suppressor T-cells), thymocytes and NK-cells. The majority of CD8+ T-cells express CD8 as heterodimer. Some subpopulations of CD8+ T-cells as well NK-cells may express homodimer. CD8 functions as a co-receptor in concert with TCR for binding the MHC class I/peptide complex. HIV-2 envelope glycoprotein binds CD8 α chain but notCD8β chain.

Species reactivity:
Positive: human.

Applications:
Characterization of leukemia and lymphoma.
ELISA
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: -

Positive control: HuT78 or hPBL, tonsil.

References:
- Leerling et al, Communications RIV (1985).
- Jonker et al, Transplantation45: 677 (1988)