CD90 (clone EBS-CD-043), anti-human, monkey

Source:
A BALB/c mouse was immunized with purified human brain Thy-1.
Fusion partner: NS-1.



Specifications:
CD90 (Thy-1) is an 18-35 kDa GPI-anchored glycoprotein and a member of the immunoglobulin superfamily. It may contribute to inhibition of proliferation/differentiation of hematopoietic stem cells and neuron memory formation in the CNS. It consists of a single Ig domain (112 amino acids; 25-35 kDa) inserted into the cell membrane via a GPI-anchor. Expressed by hematopoietic stem cells and neurons in all species studied. Its highly expressed in connective tissue and various fibroblast and stromal cell lines, expressed on all thymocytes and peripheral T-cell in mice, but in humans expressed only on small % fetal thymocytes, 10-40% of CD34+ cells in bone marrow, and <1% of lymph node HEV endothelium but not on other endothelia. Lastly, it is expressed by a limited number of lymphoblastoid and leukemic cell lines.

Species reactivity:
Positive: human, monkey.

Applications:
CD90 mAbs are very useful for identifying CD34+ hematopoietic precursor cells. CD34+ and CD90+ cells include hematopoietic stem cells that can serve as autologous grafts to replace the bone marrow in patients with malignancies.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: IMR-32, CCRF-CEM or MOLT-4 cells. Human uterus.

References:
- Cervelló I et al. PLoS One6: e21221 (2011).
- Lehmann GM et al. Am J Physiol Cell Physiol299: C672-81 (2010)