CD95 (Fas) (clone B-R18), anti-human

Source:
A BALB/c mouse was immunized with recombinant Fas antigen.
Fusion partner: P3-X63-Ag8.653.



Specifications:
B-R18 specifically recognizes CD95. CD95 is a cell surface glycoprotein with a MW of 40-45 kDa and contains 8 kDa of N-glyosidic-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. CD95 is preferentially expressed by the CD54RAlow and CD45ROhighsubset of memory T-cells, but it also found on peripheral monocytes. It is further found on human B-cell lines like pre-B cells, EBV infected cells, Burkitt cells and plasmacytoma cells. It also binds to human T-cell lines, myeloid cell lines, hepatocyte carcinoma and endothelial cells.

Species reactivity:
Positive: human.

Applications:
Applicable in frozen sections and in flow cytometry.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: MCF-7 cells; hepatocellular or bladder carcinoma.

References:
- Sasaki et al. Br J Urol 81: 852, (1998).
- Sugihara et al. Anticancer Res 17: 3861, (1997).
- Kondo et al. J Pathol 183: 75, (1997).