CD98 (4F2) (clone IPO-T10), anti-human

Source:
A BALB/c mouse was immunized with stimulated human PBL.
Fusion partner: P3-X63-Ag8.653.



Specifications:
CD98 exists as a heterodimer containing a disulphide-linked glycosylated heavy chain and a non-glycosylated light chain. It is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through disulphide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intercellular calcium levels and transport L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized. Monocytes express high levels of CD98 antigen. Peripheral blood T- and B-cells, as well as NK-cells and granulocytes express low levels of CD98. Activation of T-cells and NK-cells leads to upregulation of CD98. RBCs are negative. IPO-T10 was typed at the VIth International Workshop and Conference on Human Leukocyte Differentiation Antigens.

Species reactivity:
Positive: human.

Applications:
Applicable in frozen sections and in flow cytometry.
Flow cytometry: +
Frozen sections: +

Positive control: Jurkat, MG63, HUT-78, K562, YT, U937 and human lymphocytes or tonsils.

References:
- The VIth International Workshop and Conference on Human Leukocyte Differentiation Antigens, Kobe, Japan (1996) (Garland Publishing, Inc, London).
- Woodhead VE et al. Int Immunol.12(7):1051-61 (2000).
- Stonehouse TJ et al. Immunology 96: 35–47 (1999)