CD99 (E2, MIC2) (clone EBS-CD-044), anti-human

Source:
A BALB/c mouse was immunized with purified E-rosette forming cells from human PBL.



Specifications:
EBS-CD-044 reacts with CD99 or MIC2. Human thymocytes, PBLs and some T-ALL isolates and cell lines are positive. It is also present on pancreas and on Ewing sarcoma, which forms the practical application of the antibody. It is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes.

Species reactivity:
Positive: human.

Applications:
Immunohistochemistry on frozen sections and paraffin sections. Flow cytometry, immunofluorescence and western blotting used to identify Ewing sarcoma in tissue sections.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: Pancreas, thymus, Ewing sarcoma.

References:
- Rajasekariah et al. in: Leukocyte Typing III A. McMichael (ed), Oxford University Press, Oxford (1987).
- Sandrin et al. Immunogenetics 35: 283-28 (1992)