CDI-Activated Separopore® 4B
€0.00
In stock
SKU
BW-20181002
Highlights:
Pre-activated medium for immobilization of ligands containing primary amines.
N, N'-Carbonyl diimidazole (CDI) mediated activation of Separopore® 4B matrix to allow coupling of the primary amino ligands by a stable amide bond formation.
No charge is introduced into the matrix after the coupling reaction.
No ligand leakage after coupling.
Superior stability: Activated matrix is much more resistant to hydrolysis.
Ideal for immobilization of large protein ligands such as antibodies as well as peptides and nucleic acids.
Coupling reaction can also be carried out in organic solvents.
High coupling efficiency and easy to handle.
CDI-activated Separopore® 4B is activated with CDI groups for covalent immobilization of proteins and other molecules that contain N-nucleophiles.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Group to be coupled: -NH2 groups
Matrix: Separopore® 4B (agarose beads, 4%)
Particle size range: 52 – 165 μm
Molecular weight range: 6 x 104 - 2 x 107
Coupling conditions: pH: 8.5 – 11, Temp: 4 – 25 °C, Time: 6 – 24 h
pH stability: 11 (ligand dependent)
Storage: 2 – 8 °C
Supplied as suspension in acetone
References:
Affinity purification of plasma proteins: characterization of six affinity matrices and their application for the isolation of human factor VIII. Thromb Haemost. (1989) 61: 234-7.
Homogeneous bivalent insulin receptor: purification using insulin coupled to 1, 1'-carbonyldiimidazole activated-agarose. Biochem Biophys Res Commun. (1985) 132: 1059-65.
Related products:
CDI-activated Separopore® 6B-CL (SKU # 20181084)
CH-activated Separopore® 4B (SKU # 20181085)
CNBr-activated Separopore® 4B (SKU # 20181003)
CNBr-activated Separopore® 6B (SKU # 20181062)
Epoxy-activated Separopore® 4B-CL (SKU # 20181009)
Epoxy-activated Separopore® 6B-CL (SKU # 20181064)
NHS-activated Separopore® 4B-CL (SKU # 20181025)
Tosyl-activated Separopore® 4B-CL (SKU # 20181039)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
Pre-activated medium for immobilization of ligands containing primary amines.
N, N'-Carbonyl diimidazole (CDI) mediated activation of Separopore® 4B matrix to allow coupling of the primary amino ligands by a stable amide bond formation.
No charge is introduced into the matrix after the coupling reaction.
No ligand leakage after coupling.
Superior stability: Activated matrix is much more resistant to hydrolysis.
Ideal for immobilization of large protein ligands such as antibodies as well as peptides and nucleic acids.
Coupling reaction can also be carried out in organic solvents.
High coupling efficiency and easy to handle.
CDI-activated Separopore® 4B is activated with CDI groups for covalent immobilization of proteins and other molecules that contain N-nucleophiles.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Group to be coupled: -NH2 groups
Matrix: Separopore® 4B (agarose beads, 4%)
Particle size range: 52 – 165 μm
Molecular weight range: 6 x 104 - 2 x 107
Coupling conditions: pH: 8.5 – 11, Temp: 4 – 25 °C, Time: 6 – 24 h
pH stability: 11 (ligand dependent)
Storage: 2 – 8 °C
Supplied as suspension in acetone
References:
Affinity purification of plasma proteins: characterization of six affinity matrices and their application for the isolation of human factor VIII. Thromb Haemost. (1989) 61: 234-7.
Homogeneous bivalent insulin receptor: purification using insulin coupled to 1, 1'-carbonyldiimidazole activated-agarose. Biochem Biophys Res Commun. (1985) 132: 1059-65.
Related products:
CDI-activated Separopore® 6B-CL (SKU # 20181084)
CH-activated Separopore® 4B (SKU # 20181085)
CNBr-activated Separopore® 4B (SKU # 20181003)
CNBr-activated Separopore® 6B (SKU # 20181062)
Epoxy-activated Separopore® 4B-CL (SKU # 20181009)
Epoxy-activated Separopore® 6B-CL (SKU # 20181064)
NHS-activated Separopore® 4B-CL (SKU # 20181025)
Tosyl-activated Separopore® 4B-CL (SKU # 20181039)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
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