CDw75 (clone LN-1), anti-human

Source:
A BALB/c mouse was immunized with nuclei from pokeweed mitrogen-stimulated PBL.
Fusion partner: NS-1.



Specifications:
LN-1 reacts with CDw75, a neuraminidase sensitive cell surface sialoglycan which is present on the cell membrane and cytoplasm of germininal center B-cells and derived lymphomas. CDw75 has a function in cell adhesion and is the ligand for CD22. LN-1 reacts with RBC precursors in bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with gioblastomas and astrocytoma’s, and also Reed Sternberg cells in Hodgkin’s disease.

Species reactivity:
Positive: human.

Applications:
LN-1 can be useful in identifying B-cell phenotype and show Reed Sternberg cells in Hodgkin’s disease.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: HeLa or Daudi cells. Germinal center B-cell in a lymph node or tonsil. Use LN-1’s ability to stain the surface of erythrocytes as an internal Positive control: .

References:
- Epstein AL et. al. J of Immunology 133: 1028-1036 (1984).
- Marder RJ et. al. Lab Invest 52: 497-504 (1985).