CDw78 (MHC II DR) (clone 60-3G2), anti-human

Source:
A BALB/c mouse was immunized with human B-cell line Daudi.
Fusion partner: P3-X63-Ag8.653.



Specifications:
CDw78 (also called Ba antigen, Leu21 or LO panB a) is present on some immature and some mature B-cells. The antigen appears on B-cell progenitors preceding CD10, CD19, CD22, and CD37. It is expressed on resting B-cells and reappears and persists in the cytoplasm and on the cell surface until cytoplasmic Ig appears. Its expression is greatly increased after B-cell activation in vitro. It is also found on tissue macrophages and on epithelial cells, but not on T-cells, NK cells, monocytes, granulocytes, thymocytes or bone marrow stromal fibroblasts nor myeloid tissues. 60-3G2 was typed at CD workshop IV.

Species reactivity:
Positive: human.

Applications:
60-3G2 labels B-cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70% of B-cell CLL and some B-NHL were also positive. 60-3G2 reacts with AML cells and in a majority of Hodgkin’s disease cases a significant percentage of affected lymph node cells were detected.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: Daudi cells, Raji, Namalva, EB-3, RPM1-8226 (50% of cells), tonsil or lymph node.

References:
- Pinchouk VG. et al, Anticancer Research8: 1377-1380 (1988).
- Gluzman DF. et al, Tissue Antigens33: 151 (1989).
- Sidorenko SP. et al, Neoplasma39: 3-9 (1992).
- Moldenhauer et al, Leucocyte Typing IV, pp 155 162, (1989).
- Pezzuto et al, Leucocyte Typing IV, pp 165 174, (1989).