CDw78 (MHC II DR) (clone IPO-10), anti-human

Source:
A BALB/c mouse was immunized with human B-cell line Daudi.
Fusion partner: P3-X63-Ag8.653.



Specifications:
CDw78 (also called Ba antigen, Leu21 or LO panB a) is present on some immature and some mature B-cells. The antigen appears on B-cell progenitors preceding CD10, CD19, CD22 and CD37. It is expressed on resting B-cells and reappears and persists in the cytoplasm and on the cell surface until cytoplasmic Ig appears. Its expression is greatly increased after B-cell activation in vitro. It is also found on tissue macrophages and on epithelial cells, but not on T-cells, NK-cells, monocytes, granulocytes, thymocytes or bone marrow stromal fibroblasts nor myeloid tissues.

Species reactivity:
Positive: human.

Applications:
IPO-10 labels B-cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70% of B-cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkin’s disease cases a significant percentage of affected lymph node cells were detected.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: Daudi cells, Raji, Namalva, EB-3, RPMI-8226 (50% of cells), tonsil or lymph node.

References:
- Pinchouk V.G. et al, Anticancer Res.8:1377-1380 (1988).
- Gluzman D.F. et al, Tissue Antigens33: 151 (1989).
- Sidorenko S.P. et al, Neoplasma39: 3-9 (1992)