Chlamydia & Chlamydophila (multi epitope) (clone ACI, ICK, 502) FITC, anti-bacteria

Background:
Chlamydia is a genus of bacteria that are obligate intracellular parasites. Chlamydia infections are the most common bacterial sexually transmitted diseases in humans and are the leading cause of infectious blindness

Decription:
Mouse monoclonal antibody to Chlamydia & Chlamydophila (multi epitope) FITC conjugate

Clone: ACI, ICK, 502

Isotype: Mouse IgG3

Immunogen: Chlamydia Antigen

Reactivity: chlamydia LPS, all species

Specificity: The multi-epitope cocktail detects the elementary bodies (Ebs), reticulate bodies (RBs), intermediate forms, chlamydial inclusions and specific cell-associated antigen(s) di- rectly in samples. The conjugate is used for direct immunofluorescence staining com- bining three specific monoclonal antibodies (mab) conjugated to fluorescein isothiocy- anate (FITC). One mab is specific for the genus-specific epitope located on the Chla- mydia LPS and identifies all the 15 known serovars of C. trachomatis as well as C. psittaci and C. pneumoniae by displaying bright fluorescence of intracellular inclu- sions and pin-point shaped extracellular organisms as well as free cell-associated chlamydial antigen(s). A second mab reacts with an epitope on the Chlamydia-outer membrane complex protein (60 kD) and shows preferential fluorescence around the periphery of individual RBs of all serotypes of C. trachomatis and of C. psittaci strains; this antibody also stains the EBs of C-complex serovars as pin-point shaped structures. The third mab identifies a species-specific epitope on the major outer membrane pro- tein (40 kD) and shows brilliant fluorescence with EBs, RBs and cytoplasmic inclusions of B-complex serovars of C. trachomatis, whereas C-complex serovars show medium to weak fluorescence. The combination of these 3 specific mabs provides uniform and intense staining of all the stages in the developmental cycle of the known C. trachomatis serovars (A–C, D–K, L1–L3) and C. psittaci and C. pneumoniae strains. A positive cross-reactivity was also reported for Chlamydophila pecorum.

Form: Lyophilized FITC conjugate, in protein stabilizer, 0.09% Sodium Azide, Evans blue as counterstein

Applications: Immunofluorescence microscopy. Detects chlamydia species in clinical specimens after fixation with methanol/acetone (1:1). Also suitable for paraformaldehyde-fixed tissue sections and cell culture. Use 30 µl per test.

Reconstitution: 1.5 ml sterile distilled water

Storage: 2-8C, avoid freezing temperature