Chlamydia trachomatis serovar "L" PCR Kit RUO
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In stock
SKU
RealCycler CHSL-
Catalog Number: RealCycler CHSL-
1 Intended Use:
RealCycler CHSL is a kit of reagents which allows real-time PCR qualitative detection of Chlamydia trachomatis serovar L (lymphogranuloma venereum causal agent) DNA in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of Chlamydia trachomatis serovar L is detected in the corresponding channel of FAM fluorophore and CHIC is detected in the corresponding channel of Alx532 or HEX fluorophore.
3 Technical specifications
Sensitivity
- Chlamydia trachomatis serovar L: 100 copies/μL.
Specificity
- Chlamydia trachomatis serovar L (pmpH gene).
4 Contents
RealCycler CHSL-U / CHSL-G includes the AmpliMix and a LGV DNA Positive Control.
All reagents are ready to use without adding or rebuilding any component.
RealCycler CHSL is a kit of reagents which allows real-time PCR qualitative detection of Chlamydia trachomatis serovar L (lymphogranuloma venereum causal agent) DNA in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of Chlamydia trachomatis serovar L is detected in the corresponding channel of FAM fluorophore and CHIC is detected in the corresponding channel of Alx532 or HEX fluorophore.
3 Technical specifications
Sensitivity
- Chlamydia trachomatis serovar L: 100 copies/μL.
Specificity
- Chlamydia trachomatis serovar L (pmpH gene).
4 Contents
RealCycler CHSL-U / CHSL-G includes the AmpliMix and a LGV DNA Positive Control.
All reagents are ready to use without adding or rebuilding any component.
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