Clostridium difficile toxin A (clone EBS-I-100), anti-C. difficile.

Source:
A BALB/c mouse was immunized with C. difficiletoxin A.
Fusion partner: Sp2/0.

Specifications:
EBS-I-100 reacts with C. difficile Toxin A, but not with V. cholerae subunit a, V. cholerae toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. C. difficile is a major nosocomial pathogen that causes antibiotic-associated colitis and mediates inflammatory diarrhea by releasing two large protein enterotoxins (toxin A and toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. C. difficile toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. C. difficile toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.

Species reactivity:
Positive: C. difficile. Negative: V. cholera, Pseudomonas aeruginosa.

Applications:
Test for presence of C. difficile toxin A in native samples. No heat treatment necessary.
ELISA: +
Frozen sections: +
Immunofluorescence: +

Positive control: Clostridium difficile extract or infected cells or tissue.

References:
- Kim H, et al,Gastroenterology129: 1875-1888 (2005).
- Carter JP, et al, Gut Microbes.1(1): 58–64 (2010)