CNBr-Activated Separopore® 4B
€0.00
In stock
SKU
BW-20181003
Application:
Cyanogen bromide-activated Separopore® 4B matrix is used for preparation of resins for affinity chromatography.
Pre-activated medium is suitable for immobilization of ligands containing primary amines.
Cyanogen bromide (CNBr) in base reacts with hydroxyl groups on Separopore® 4B to form cyanate esters or imidocarbonates.
These groups react readily with primary amines under very mild conditions; the net result is a covalent coupling of a ligand to the Separopore® matrix.
Highlights:
Reproducible, simple and reliable method for covalent immobilization of ligands containing primary amines.
Multi-point attachment of many protein ligands, resulting in a chemically stable product.
Mild coupling condition makes CNBr-activated matrix as the ideal tool for coupling enzymes and antibodies.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Group to be coupled: -NH2
Matrix: Separopore® 4B (agarose beads, 4%)
Particle size range: 52 – 165 μm
Molecular weight range: 6 x 104 – 2 x 107
Binding capacity: 10 – 25 mg α-chymotrypsinogen / ml drained gel
pH Stability: 2 – 11 (ligand dependent)
Coupling conditions: pH 9 – 10, Temp: 4 – 25 ºC, Time: 4 – 16 h
Storage: 2 – 8 °C
Supplied as lyophilized powder (with additives for stabilization)
1g swell to ~ 5 ml
References: Novel extraction supports based on immobilised aptamers: Evaluation for the selective extraction of cocaine. Talanta. (2011) 85: 616-24.
New approach to quantitative analysis of benzo[a]pyrene in food supplements by an immunochemical column test. Talanta. (2011) 85: 151-6.
Immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of Paramphistomum epiclitum. J Parasit Dis. (2010) 34: 57-61.
Protein hydrolysis by immobilized and stabilized trypsin. Biotechnol Prog. (2011) 27: 677-83.
Preparation and characterization of an immunoaffinity column for the selective extraction of salbutamol from pork sample. J Chromatogr Sci. (2011) 49: 276-80.
A proteome-scale study on in vivo protein Nα-acetylation using an optimized method. Proteomics. (2011) 11: 81-93.
Molecular cloning and biochemical characterization of a myotoxin inhibitor from Bothrops alternatus snake plasma. Biochimie. (2011) 93: 583-92.
Establishment of an immunoaffinity chromatography for simultaneously selective extraction of Sudan I, II, III and IV from food samples. J Chromatogr A. (2010) 1217: 7840-7.
Quantitative detection of trace systemins in Solanaceous plants by immunoaffinity purification combined with liquid chromatography/electrospray quadrupole time-of-flight mass spectrometry. Anal Chem. (2010) 82: 9374-83.
Epigallocatechin-3-gallate suppresses the expression of HSP70 and HSP90 and exhibits anti-tumor activity in vitro and in vivo. BMC Cancer. (2010) 10: 276.
Determination of epitestosterone in human urine by off-line immunoaffinity solid-phase extraction coupled with high performance liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. (2010) 878: 1443-8.
One-step purification of vitronectin from human plasma by affinity chromatography on phage-displayed peptides. Acta Biochim Pol. (2010) 57: 89-93.
Preparation and characterization of an immunoaffinity chromatography column for the selective extraction of trace contraceptive drug levonorgestrel from water samples. Talanta. (2009) 80: 98-103.
A proteomics approach to study in vivo protein N(alpha)-modifications. J Proteomics. (2009) 73: 240-51.
Purification process development for HER1 extracellular domain as a potential therapeutic vaccine. J Chromatogr B Analyt Technol Biomed Life Sci. (2009) 877: 3105-10.
Preparation of acetochlor antibody and its application on immunoaffinity chromatography cleanup for residue determination in peanuts. J Agric Food Chem. (2009) 57: 7640-3.
Determination of cocaine in human plasma by selective solid-phase extraction using an aptamer-based sorbent. Anal Chem. (2009) 81: 7081-6.
Flow injection analysis of angiotensin I-converting enzyme inhibitory activity with enzymatic reactors. Talanta. (2009) 79: 1130-4.
Immunoglobulin G-reactive antibodies from sera of healthy individuals enriched in IgG2--therapeutic potential in HIV-1 infection. Curr HIV Res. (2009) 7: 378-83.
Purification of nine sulfonamides from chicken tissues by immunoaffinity chromatography using two monoclonal antibodies. J AOAC Int. (2008) 91: 1488-93.
Determination of microcystin-LR in water from Lake Tai, China. Bull Environ Contam Toxicol. (2009) 82: 230-3.
Evaluation of various immobilized enzymatic microreactors coupled on-line with liquid chromatography and mass spectrometry detection for quantitative analysis of cytochrome c. J Chromatogr A. (2008) 1209: 95-103.
Assessment of protein phosphatase in a re-usable rapid assay format in detecting microcystins and okadaic acid as a precursor to biosensor development. Toxicon. (2008) 52: 745-53.
Purification of sequence-specific DNA-binding proteins by affinity chromatography. Curr Protoc Mol Biol. (2001) Chapter 12: Unit 12.10.
A procedure for the rapid screening of Maillard reaction inhibitors. J Biochem Biophys Methods. (2008) 70: 958-65.
Androgen receptor coactivator ARA70alpha and ARA70beta isoform-specific antibodies: new tools for studies of expression and immunohistochemical localization. Appl Immunohistochem Mol Morphol. (2008) 16: 7-12.
Purification of CK1 by affinity chromatography on immobilised axin. Protein Expr Purif. (2007) 54: 101-9.
Simultaneous non-instrumental detection of aflatoxin B1 and ochratoxin A using a clean-up tandem immunoassay column. Anal Chim Acta. (2007) 590: 118-24.
Stem bromelain: an enzyme that naturally facilitates oriented immobilization. Protein Pept Lett. (2007) 14: 233-6.
Multiresidue determination of zeranol and related compounds in bovine muscle by gas chromatography/mass spectrometry with immunoaffinity cleanup. J AOAC Int. (2006) 89: 1677-81.
Isolation and partial characterization of ribonuclease inhibitor from goat liver. Protein Pept Lett. (2006) 13: 779-83.
Bioaffinity based oriented immobilization of stem bromelain. Biotechnol Lett. (2006) 28: 917-22.
Characterization of calcineurin-dependent response element binding protein and its involvement in copper-metallothionein gene expression in Neurospora. Biochem Biophys Res Commun. (2006) 345: 1010-3.
Residue analysis for halofuginone in sturgeon muscle by immunoaffinity cleanup and liquid chromatography. J AOAC Int. (2005) 88: 1644-8.
Development and application of a multi-target immunoaffinity column for the selective extraction of natural estrogens from pregnant women's urine samples by capillary electrophoresis. J Chromatogr B Analyt Technol Biomed Life Sci. (2005) 816: 7-14.
The critical concentration of C1-esterase inhibitor (C1-INH) in human serum preventing auto-activation of the first component of complement (C1). Mol Immunol. (2005) 42: 657-63.
Application of immunoaffinity column as cleanup tool for an enzyme linked immunosorbent assay of phosphinothricin-N-acetyltransferase detection in genetically modified maize and rape. J Agric Food Chem. (2005) 53: 4315-21.
Selective depletion of glycyrrhizin from Si-Ni-San, a traditional Chinese prescription, blocks its effect on contact sensitivity in mice and recovers adhesion and metalloproteinases production of T lymphocytes. Int Immunopharmacol. (2005) 5: 1193-204.
Analysis of proteome bound to D-loop region of mitochondrial DNA by DNA-linked affinity chromatography and reverse-phase liquid chromatography/tandem mass spectrometry. Ann N Y Acad Sci. (2005) 1042: 88-100.
High-performance catalytic chromatography. The adapter approach. J Chromatogr A. (2005) 1076: 71-82.
An efficient immunoaffinity chromatographic method for extraction and purification of papaverine from samples of pericarpium papaveris and food products. J Sep Sci. (2005) 28: 1163-70.
Multiresidue analysis of avermectins in bovine liver by immunoaffinity column cleanup procedure and liquid chromatography with fluorescence detector. J AOAC Int. (2005) 88: 1099-103.
Related products:
CDI-activated Separopore® 4B (SKU # 20181002)
CDI-activated Separopore® 6B-CL (SKU # 20181084)
CH-activated Separopore® 4B (SKU # 20181085)
CNBr-activated Separopore® 6B (SKU # 20181062)
Epoxy-activated Separopore® 4B-CL (SKU # 20181009)
Epoxy-activated Separopore® 6B-CL (SKU # 20181064)
NHS-activated Separopore® 4B-CL (SKU # 20181025)
Tosyl-activated Separopore® 4B-CL (SKU # 20181039)
Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping : Non-hazardous
Storage : -20°C
Appearance Form : Powder
Appearance Color : White
Brand Name : bioPLUS™, Separopore®
Cyanogen bromide-activated Separopore® 4B matrix is used for preparation of resins for affinity chromatography.
Pre-activated medium is suitable for immobilization of ligands containing primary amines.
Cyanogen bromide (CNBr) in base reacts with hydroxyl groups on Separopore® 4B to form cyanate esters or imidocarbonates.
These groups react readily with primary amines under very mild conditions; the net result is a covalent coupling of a ligand to the Separopore® matrix.
Highlights:
Reproducible, simple and reliable method for covalent immobilization of ligands containing primary amines.
Multi-point attachment of many protein ligands, resulting in a chemically stable product.
Mild coupling condition makes CNBr-activated matrix as the ideal tool for coupling enzymes and antibodies.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Group to be coupled: -NH2
Matrix: Separopore® 4B (agarose beads, 4%)
Particle size range: 52 – 165 μm
Molecular weight range: 6 x 104 – 2 x 107
Binding capacity: 10 – 25 mg α-chymotrypsinogen / ml drained gel
pH Stability: 2 – 11 (ligand dependent)
Coupling conditions: pH 9 – 10, Temp: 4 – 25 ºC, Time: 4 – 16 h
Storage: 2 – 8 °C
Supplied as lyophilized powder (with additives for stabilization)
1g swell to ~ 5 ml
References: Novel extraction supports based on immobilised aptamers: Evaluation for the selective extraction of cocaine. Talanta. (2011) 85: 616-24.
New approach to quantitative analysis of benzo[a]pyrene in food supplements by an immunochemical column test. Talanta. (2011) 85: 151-6.
Immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of Paramphistomum epiclitum. J Parasit Dis. (2010) 34: 57-61.
Protein hydrolysis by immobilized and stabilized trypsin. Biotechnol Prog. (2011) 27: 677-83.
Preparation and characterization of an immunoaffinity column for the selective extraction of salbutamol from pork sample. J Chromatogr Sci. (2011) 49: 276-80.
A proteome-scale study on in vivo protein Nα-acetylation using an optimized method. Proteomics. (2011) 11: 81-93.
Molecular cloning and biochemical characterization of a myotoxin inhibitor from Bothrops alternatus snake plasma. Biochimie. (2011) 93: 583-92.
Establishment of an immunoaffinity chromatography for simultaneously selective extraction of Sudan I, II, III and IV from food samples. J Chromatogr A. (2010) 1217: 7840-7.
Quantitative detection of trace systemins in Solanaceous plants by immunoaffinity purification combined with liquid chromatography/electrospray quadrupole time-of-flight mass spectrometry. Anal Chem. (2010) 82: 9374-83.
Epigallocatechin-3-gallate suppresses the expression of HSP70 and HSP90 and exhibits anti-tumor activity in vitro and in vivo. BMC Cancer. (2010) 10: 276.
Determination of epitestosterone in human urine by off-line immunoaffinity solid-phase extraction coupled with high performance liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. (2010) 878: 1443-8.
One-step purification of vitronectin from human plasma by affinity chromatography on phage-displayed peptides. Acta Biochim Pol. (2010) 57: 89-93.
Preparation and characterization of an immunoaffinity chromatography column for the selective extraction of trace contraceptive drug levonorgestrel from water samples. Talanta. (2009) 80: 98-103.
A proteomics approach to study in vivo protein N(alpha)-modifications. J Proteomics. (2009) 73: 240-51.
Purification process development for HER1 extracellular domain as a potential therapeutic vaccine. J Chromatogr B Analyt Technol Biomed Life Sci. (2009) 877: 3105-10.
Preparation of acetochlor antibody and its application on immunoaffinity chromatography cleanup for residue determination in peanuts. J Agric Food Chem. (2009) 57: 7640-3.
Determination of cocaine in human plasma by selective solid-phase extraction using an aptamer-based sorbent. Anal Chem. (2009) 81: 7081-6.
Flow injection analysis of angiotensin I-converting enzyme inhibitory activity with enzymatic reactors. Talanta. (2009) 79: 1130-4.
Immunoglobulin G-reactive antibodies from sera of healthy individuals enriched in IgG2--therapeutic potential in HIV-1 infection. Curr HIV Res. (2009) 7: 378-83.
Purification of nine sulfonamides from chicken tissues by immunoaffinity chromatography using two monoclonal antibodies. J AOAC Int. (2008) 91: 1488-93.
Determination of microcystin-LR in water from Lake Tai, China. Bull Environ Contam Toxicol. (2009) 82: 230-3.
Evaluation of various immobilized enzymatic microreactors coupled on-line with liquid chromatography and mass spectrometry detection for quantitative analysis of cytochrome c. J Chromatogr A. (2008) 1209: 95-103.
Assessment of protein phosphatase in a re-usable rapid assay format in detecting microcystins and okadaic acid as a precursor to biosensor development. Toxicon. (2008) 52: 745-53.
Purification of sequence-specific DNA-binding proteins by affinity chromatography. Curr Protoc Mol Biol. (2001) Chapter 12: Unit 12.10.
A procedure for the rapid screening of Maillard reaction inhibitors. J Biochem Biophys Methods. (2008) 70: 958-65.
Androgen receptor coactivator ARA70alpha and ARA70beta isoform-specific antibodies: new tools for studies of expression and immunohistochemical localization. Appl Immunohistochem Mol Morphol. (2008) 16: 7-12.
Purification of CK1 by affinity chromatography on immobilised axin. Protein Expr Purif. (2007) 54: 101-9.
Simultaneous non-instrumental detection of aflatoxin B1 and ochratoxin A using a clean-up tandem immunoassay column. Anal Chim Acta. (2007) 590: 118-24.
Stem bromelain: an enzyme that naturally facilitates oriented immobilization. Protein Pept Lett. (2007) 14: 233-6.
Multiresidue determination of zeranol and related compounds in bovine muscle by gas chromatography/mass spectrometry with immunoaffinity cleanup. J AOAC Int. (2006) 89: 1677-81.
Isolation and partial characterization of ribonuclease inhibitor from goat liver. Protein Pept Lett. (2006) 13: 779-83.
Bioaffinity based oriented immobilization of stem bromelain. Biotechnol Lett. (2006) 28: 917-22.
Characterization of calcineurin-dependent response element binding protein and its involvement in copper-metallothionein gene expression in Neurospora. Biochem Biophys Res Commun. (2006) 345: 1010-3.
Residue analysis for halofuginone in sturgeon muscle by immunoaffinity cleanup and liquid chromatography. J AOAC Int. (2005) 88: 1644-8.
Development and application of a multi-target immunoaffinity column for the selective extraction of natural estrogens from pregnant women's urine samples by capillary electrophoresis. J Chromatogr B Analyt Technol Biomed Life Sci. (2005) 816: 7-14.
The critical concentration of C1-esterase inhibitor (C1-INH) in human serum preventing auto-activation of the first component of complement (C1). Mol Immunol. (2005) 42: 657-63.
Application of immunoaffinity column as cleanup tool for an enzyme linked immunosorbent assay of phosphinothricin-N-acetyltransferase detection in genetically modified maize and rape. J Agric Food Chem. (2005) 53: 4315-21.
Selective depletion of glycyrrhizin from Si-Ni-San, a traditional Chinese prescription, blocks its effect on contact sensitivity in mice and recovers adhesion and metalloproteinases production of T lymphocytes. Int Immunopharmacol. (2005) 5: 1193-204.
Analysis of proteome bound to D-loop region of mitochondrial DNA by DNA-linked affinity chromatography and reverse-phase liquid chromatography/tandem mass spectrometry. Ann N Y Acad Sci. (2005) 1042: 88-100.
High-performance catalytic chromatography. The adapter approach. J Chromatogr A. (2005) 1076: 71-82.
An efficient immunoaffinity chromatographic method for extraction and purification of papaverine from samples of pericarpium papaveris and food products. J Sep Sci. (2005) 28: 1163-70.
Multiresidue analysis of avermectins in bovine liver by immunoaffinity column cleanup procedure and liquid chromatography with fluorescence detector. J AOAC Int. (2005) 88: 1099-103.
Related products:
CDI-activated Separopore® 4B (SKU # 20181002)
CDI-activated Separopore® 6B-CL (SKU # 20181084)
CH-activated Separopore® 4B (SKU # 20181085)
CNBr-activated Separopore® 6B (SKU # 20181062)
Epoxy-activated Separopore® 4B-CL (SKU # 20181009)
Epoxy-activated Separopore® 6B-CL (SKU # 20181064)
NHS-activated Separopore® 4B-CL (SKU # 20181025)
Tosyl-activated Separopore® 4B-CL (SKU # 20181039)
Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping : Non-hazardous
Storage : -20°C
Appearance Form : Powder
Appearance Color : White
Brand Name : bioPLUS™, Separopore®
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