CNBr-Activated Separopore® 6B
€0.00
In stock
SKU
BW-20181062
Application:
Cyanogen bromide-activated Separopore® 6B matrix is used for preparation of resins for affinity chromatography. Pre-activated medium is suitable for immobilization of ligands containing primary amines. Cyanogen bromide (CNBr) in base reacts with hydroxyl groups on Separopore® to form cyanate esters or imidocarbonates. These groups react readily with primary amines under very mild conditions; the net result is a covalent coupling of a ligand to the Separopore® matrix.
Highlights:
Reproducible, simple and reliable method for covalent immobilization of ligands containing primary amines.
Multi-point attachment of many protein ligands, resulting in a chemically stable product.
Mild coupling condition makes CNBr-activated matrix as the ideal tool for coupling enzymes and antibodies.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Group to be coupled: -NH2
Matrix: Separopore® 6B (agarose beads, 6%)
Particle size range: 52 – 165 μm
Molecular weight range: 6 x 104 - 2 x 107
Binding capacity: 10 - 25 mg α-chymotrypsinogen / ml drained gel
pH Stability: 2-11 (ligand dependent)
Coupling conditions: pH 9 – 10, Temp: 4 – 25 ºC, Time: 4 – 16 h
Supplied as lyophilized powder (with additives for stabilization)
1 g swell to ~ 5 ml
References:
Studies of the distribution of proteins bound to CNBr-activated Sepharose 6B at the electron-microscopic level. Eur J Biochem.(1975) 60: 163-7.
Isolation and some properties of immobilized cystathionine-beta-synthase. Biokhimiia. (1980) 45: 1960-3.
Related products:
CDI-activated Separopore® 4B (SKU # 20181002)
CDI-activated Separopore® 6B-CL (SKU # 20181084)
CH-activated Separopore® 4B (SKU # 20181085)
CNBr-activated Separopore® 4B (SKU # 20181003)
Epoxy-activated Separopore® 4B-CL (SKU # 20181009)
Epoxy-activated Separopore® 6B-CL (SKU # 20181064)
NHS-activated Separopore® 4B-CL (SKU # 20181025)
Tosyl-activated Separopore® 4B-CL (SKU # 20181039)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: -20°C
Brand Name: bioPLUS™, Separopore®
Cyanogen bromide-activated Separopore® 6B matrix is used for preparation of resins for affinity chromatography. Pre-activated medium is suitable for immobilization of ligands containing primary amines. Cyanogen bromide (CNBr) in base reacts with hydroxyl groups on Separopore® to form cyanate esters or imidocarbonates. These groups react readily with primary amines under very mild conditions; the net result is a covalent coupling of a ligand to the Separopore® matrix.
Highlights:
Reproducible, simple and reliable method for covalent immobilization of ligands containing primary amines.
Multi-point attachment of many protein ligands, resulting in a chemically stable product.
Mild coupling condition makes CNBr-activated matrix as the ideal tool for coupling enzymes and antibodies.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Group to be coupled: -NH2
Matrix: Separopore® 6B (agarose beads, 6%)
Particle size range: 52 – 165 μm
Molecular weight range: 6 x 104 - 2 x 107
Binding capacity: 10 - 25 mg α-chymotrypsinogen / ml drained gel
pH Stability: 2-11 (ligand dependent)
Coupling conditions: pH 9 – 10, Temp: 4 – 25 ºC, Time: 4 – 16 h
Supplied as lyophilized powder (with additives for stabilization)
1 g swell to ~ 5 ml
References:
Studies of the distribution of proteins bound to CNBr-activated Sepharose 6B at the electron-microscopic level. Eur J Biochem.(1975) 60: 163-7.
Isolation and some properties of immobilized cystathionine-beta-synthase. Biokhimiia. (1980) 45: 1960-3.
Related products:
CDI-activated Separopore® 4B (SKU # 20181002)
CDI-activated Separopore® 6B-CL (SKU # 20181084)
CH-activated Separopore® 4B (SKU # 20181085)
CNBr-activated Separopore® 4B (SKU # 20181003)
Epoxy-activated Separopore® 4B-CL (SKU # 20181009)
Epoxy-activated Separopore® 6B-CL (SKU # 20181064)
NHS-activated Separopore® 4B-CL (SKU # 20181025)
Tosyl-activated Separopore® 4B-CL (SKU # 20181039)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: -20°C
Brand Name: bioPLUS™, Separopore®
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