Cobalt-Chelated Separopore® 4B-CL
€0.00
In stock
SKU
BW-20181047
Application:
Co2+-chelated Separopore® 4B-CL is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to metal ions. It is suitable for both laboratory scale purification and large-scale use.
Properties:
Affinity matrix for proteins.
Imminodiacetate immobilized matrix charged with Co2+ metal ions.
Ideal for purification of recombinant proteins containing (His)6-fusion tag.
It provides effective binding and selectivity for a wide range of proteins.
High salt concentrations can be used and it withstands effective and rigorous Cleaning-In-Procedure (CIP).
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Cobalt
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 – 165 µm
Matrix activation: Epoxy
Spacer arm: 14 atoms
Ligand density: > 20 µmol Co2+ / ml drained gel
Binding capacity: ~ 5 mg histidine-tagged protein / ml drained gel
pH stability: 3 – 12
Chemical Stability: 40 °C for 7 days in 0.01M HCl, 1M NaOH, and acetate buffer pH 4, 20% ethanol
Autoclavable at 121 °C in 20% ethanol
Antimicrobial agent: 20% ethanol
Supplied as suspension in 20% ethanol
References:
Expression of truncated tobacco osmotin in Escherichia coli: purification and antifungal activity. Biotechnol Lett. (2011) 33: 539-43.
Polyethylene glycosylation prolongs the circulatory stability of recombinant human butyrylcholinesterase. Chem Biol Interact. (2005) 157-158: 115-21.
Purification and characterization of a soluble bioactive amino-terminal extracellular domain of the human thyrotropin receptor. Biochemistry. (2001) 40: 9860-9.
Related products:
Metal ion-chelating-Separopore® 4B-CL (Metal-free) (SKU # 20181020)
Cu2+-chelated Separopore® 4B-CL (SKU # 20181048)
Ni2+-chelated Separopore® 4B-CL (SKU # 20181046)
Zn2+-Chelated Separopore® 4B-CL (SKU # 20181049)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
Co2+-chelated Separopore® 4B-CL is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to metal ions. It is suitable for both laboratory scale purification and large-scale use.
Properties:
Affinity matrix for proteins.
Imminodiacetate immobilized matrix charged with Co2+ metal ions.
Ideal for purification of recombinant proteins containing (His)6-fusion tag.
It provides effective binding and selectivity for a wide range of proteins.
High salt concentrations can be used and it withstands effective and rigorous Cleaning-In-Procedure (CIP).
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Cobalt
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 – 165 µm
Matrix activation: Epoxy
Spacer arm: 14 atoms
Ligand density: > 20 µmol Co2+ / ml drained gel
Binding capacity: ~ 5 mg histidine-tagged protein / ml drained gel
pH stability: 3 – 12
Chemical Stability: 40 °C for 7 days in 0.01M HCl, 1M NaOH, and acetate buffer pH 4, 20% ethanol
Autoclavable at 121 °C in 20% ethanol
Antimicrobial agent: 20% ethanol
Supplied as suspension in 20% ethanol
References:
Expression of truncated tobacco osmotin in Escherichia coli: purification and antifungal activity. Biotechnol Lett. (2011) 33: 539-43.
Polyethylene glycosylation prolongs the circulatory stability of recombinant human butyrylcholinesterase. Chem Biol Interact. (2005) 157-158: 115-21.
Purification and characterization of a soluble bioactive amino-terminal extracellular domain of the human thyrotropin receptor. Biochemistry. (2001) 40: 9860-9.
Related products:
Metal ion-chelating-Separopore® 4B-CL (Metal-free) (SKU # 20181020)
Cu2+-chelated Separopore® 4B-CL (SKU # 20181048)
Ni2+-chelated Separopore® 4B-CL (SKU # 20181046)
Zn2+-Chelated Separopore® 4B-CL (SKU # 20181049)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
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