Cofilin Phospho-Regulation Antibody Sampler Kit

Background:
Members of the actin depolymerizing factor (ADF)/cofilin family, cofilin 1, cofilin 2, and ADF are actin-severing proteins that regulate actin remodeling during cellular events such as cell migration, cytokinesis, phagocytosis, endocytosis, axon development, and immune cell activation. Regulation of cofilin activity can occur through phosphorylation of Ser-3 by LIMK1 or LIMK2. LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK. PAK1 and ROCK phosphorylate LIMK1 or LIMK2 in the activation loop at Thr-508 or Thr-505 residues, respectively. This increases LIMK activity leading to phosphorylation of cofilin at Ser-3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. In contrast, Slingshot can dephosphorylate Ser-3 in cofilin promoting actin binding. In mammals, the Slingshot family includes SSH1L, SSH2L, and SSH3L. SSH1L and SSH2L mRNAs are widely expressed, while SSH3L has high expression in epithelial tissues. SSH1L can associate with F-actin and may be the major phosphatase regulating cofilin activity.

Buffer/Storage:
Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.