Collagen Type IV (clone 1043), anti-human, bovine, swine

Background:
The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasma. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the anti-serum is important in the differential diagnosis of bullous lesions in dermatopathology.

Clone: 1043

Isotype: Mouse IgG1

Source: 1043 is a Mouse monoclonal IgG1 antibody derived by fusion of Sp 2/0 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with purified Human type IV collagen. The immunogen has been isolated from Human placenta.

Reactivity: bovine, human, swine

Specificity: 1043 recognizes the extracellular – basement membranes. 1043 is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 .

Form: Each vial contains 100 ul 1mg/ml of purified monoclonal antibody in PBS containing 0.09% sodium azide

Applications: Flow Cytometry, Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Western blotting

Storage: Store at 4C, or in small aliquots at -20C.