CRASH (clone 7B6), anti-human

Source:
A BALB/c mouse was immunized with CRASH recombinant protein and synthetic peptide.
Fusion partner: SP2/0.

Specifications:
7B6 reacts with CRASH, a 308 AA glycoprotein sharing 32% homology with human asparaginase and identical to Asparaginase-Like Protein (ALP) found in rat sperm. CRASH is found in a variety of tumors but only occasionally in normal tissues. Especially ovarian cancers are highly positive. CRASH is further found in brain tumors, some colonic cancers, some lung cancers, some prostate cancers, uterine cancers, some breast cancers and some thyroid cancers. It was not found in serum. In Western blot 3 bands are demonstrated of 45 kDa, 28 kDa and 15 kDa.

Species reactivity:
Positive: human.

Applications:
7B6 can be used on paraffin sections, ELISA, and Western blot.
ELISA: +
Frozen sections: +
Paraffin sections: Citrate
Western blot: +

Positive control: Ovarian cancer, KM22 breast cancer cells.

References:
- Weidle, UH et al, Anticancer Res. 29(4): 951-63 (2009)