CVX5α™ Chemically Competent Cells

CVX5α™ Chemically Competent Cells is a versatile, convenient and cost-effective solution for routine subcloning procedures or any application where the starting DNA is not limiting. It is calcium chloride-treated to facilitate attachment of the plasmid DNA to the competent cell membrane.

CVX5α™ Genotype: F – , gyrA96, recA1, endA1, thi1, hsdR17 (rK – mK +), deoR, supE44, ∆ (lacZYA-argF) U169 φ80lacZ∆M15 (similar to DH5™ ).

Advantages & Features
- Versatile: proven performance for high-efficiency transformation in a wide variety of applications.
- Convenient: ideal for routine.
- Compatible: with blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal.

Specifications
CVX5α™ Genotype: F – , gyrA96, recA1, endA1, thi1, hsdR17 (rK – mK +), deoR, supE44, ∆ (lacZYA-argF) U169 φ80lacZ∆M15 (similar to DH5™ ).

Includes
Includes for 40 rxn:
- 2,000 µL CVX5α™ (1 x 107 CFU/µg)
- 10 µL pUC18 Transformation Control Plasmid (10 ng/µL)
- 50 mL SOC Medium
- Dry ice

Applications
Routine cloning and subcloning of genes into plasmid vectors.

Shipping & Storage
Shipped in: Dry ice.
Storage: Upon receipt, store cells immediately at -80 °C.
Recommendations: Storage at -20 °C will result in a significant decrease in transformation efficiency. Please note that competent cells are very sensitive to cycles of freezing and thawing and should not be exposed to temperature variations
Extra charges for dry ice apply.