CVX™ T7 ARCA mRNA Synthesis Kit

CVX™ T7 ARCA mRNA Synthesis Kit is designed for in vitro ARCA-capped (m)RNA Synthesis by T7 RNA Polymerase. The cap structure is added to the mRNA by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA). ARCA is incorporated into mRNA exclusively in the correct orientation, generating capped mRNA that is more efficiently translated.

By using a DNA template encoding a poly(A) tail, the CVX™ T7 ARCA mRNA Kit can be used to synthesize capped and tailed mRNAs.

mRNAs synthesized with the CVX™ T7 ARCA mRNA Kit can subsequently be used for cell transfection, microinjection, in vitro translation and RNA vaccines. The kit contains sufficient reagents for 50 reactions of 20 μl each. A 20 μl reaction yields about 50 μg RNA with 1 μg of template added (1.5 kb RNA transcript). The T7 RNA Polymerase Enzyme mix includes Ribonuclease Inhibitor Protein to protect newly transcribed RNA from degradation by RNases.

Advantages & Features
- High-Quality Kit: High enzymatic purity, specificity, and activity.
- Scaleable: Increase the reaction size to produce milligram amounts of RNA.
- Easy and time-saving protocol with minimal handling steps.
- Convenient: Rnase Inhibitor included.

Includes
- 3 x 40 μl T7 RNA Polymerase mix (include RNase Inhibitor)
- 200 μl T7 Reaction Buffer 10x (HEPES-based)
- 100 μl ATP (100 mM)
- 100 μl GTP (100 mM)
- 100 μl CTP (100 mM)
- 100 μl UTP (100 mM)
- 100 μl DTT (100 mM)
- 10 µl ARCA Solution (100mM)
- 10 µl T7 Control template 2 (200 ng/µl)
- 1.2 ml Nuclease-Free Water

Applications
- Synthesis of ARCA-capped (m)RNA.
- Aantisense RNA and RNAi experiments.
- RNA vaccines.
- RNA structure and function studies.

Shipping & Storage
Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).