CVX™ T7 RNA Synthesis Kit

CVX™ T7 Transcription Kit is designed for in vitro RNA synthesis by T7 RNA Polymerase from linearized plasmid DNA templates or DNA templates generated by PCR. Synthesized RNA can subsequently be used for in vitro translation, RNase protection assays, RNA splicing,hybridization assays and Northern Blot experiments.

The kit contains sufficient reagents for 50 reactions of 20 μl each. A 20 μl reaction yields about 150 μg RNA with 1 μg of template added (1.5 kb RNA transcript). The T7 RNA Polymerase Enzyme mix includes Ribonuclease Inhibitor Protein to protect newly transcribed RNA from degradation by RNases.

Advantages & Features
- High-Quality Kit: High enzymatic purity, specificity, and activity.
- Scaleable: Increase the reaction size to produce milligram amounts of RNA.
- Easy and time-saving protocol with minimal handling steps.
- Convenient: Rnase Inhibitor included.

Includes
- 3 x 40 μl T7 RNA Polymerase mix (Include RNase Inhibitor)
- 200 μl T7 Reaction Buffer 10x (HEPES-based)
- 100 μl ATP (100 mM)
- 100 μl GTP (100 mM)
- 100 μl CTP (100 mM)
- 100 μl UTP (100 mM)
- 100 μl DTT (100 mM)
- 10 µl Control template 1 (200 ng/µl)
- 10 µl Control template 2 (200 ng/µl)
- 1.2 ml Nuclease-Free Water

Applications
- In vitro Transcription.
- In vitro Translation.
- Hybridization probes generation.
- RNase protection assays.
- RNA binding Protein assays.
- Synthesis of antisense RNA.

Shipping & Storage
Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).