Cycle (appears earlier than Ki-67) (clone IPO-38), anti-human, mouse, rat

Source:
A BALB/c mouse was immunized with spleen cells of a patient with hairy cell leukemia.
Fusion partner: SP2/0.

Specifications:
IPO-38 reacts with a 12-14 kDa protein, as found in Western blots of Raji cells, and appears in the mitotic cycle earlier than Ki-67. Lymphocytes, induced to early G1 phase by 12h exposure to PHA, will become positive while non-stimulated lymphocytes remain negative. Mononuclear cells and granulocytes of healthy donors are negative, while various forms of leukemia and lymphoma including Hodgkin’s disease are positive for IPO-38, as are many solid tumors such as some breast, gastric and colonic cancers for which it may serve as tumor progression marker.

Species reactivity:
Positive: human, mouse, rat.

Applications:
IPO-38 can be used to establish the proliferation index in tissue sections of a variety of tumors, including odontogenic lesions. In addition it has been reported as a potential serum marker for gastric cancer.
ELISA: +
Frozen sections: +
Immunoprecipitation: +
Paraffin sections: Citrate
Western blot: +

Positive control: Raji cells.

References:
- Sidorenko, S.P. et al., Gematol. Transfuziol.35: 19-22 (1990).Sidorenko, S.P. et al., Experem. Oncol.16: 145-150 (1994).Thosaporn W., et al., Oral Dis. 10(1): 22-6 (2004).Hao Y. et al.,J Proteome Res.Sep;7(9): 3668-77 (2008).Makohon N.V. et al., Fiziol Zh. 54(6): 49-57 (2008).