Cytoskeletal Filament Labeling Immunocytochemistry Kit

Background:
The cytoskeleton includes actin filaments, microtubules, and intermediate filaments. These major cytoskeletal filaments are important for cell motility, cell adhesion, and cell morphology. Actin exists in two principal forms, globular, monomeric G-actin and filamentous, polymeric F-actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. Intermediate filament (IF) proteins are a diverse family with distinct expression patterns and functions. One major IF protein is vimentin, which forms filaments in mesenchymal, endothelial, and hematopoietic cells.

Buffer/Storage:
Mouse monoclonal and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.