D-Alanine Separopore® 4B-CL

Application: Used for the purification of liposomal proteins and glycopeptide antibiotics such as teicoplanin and vancomycin group.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical Specifications:
Ligand: D-Alanine
Matrix: Separopore® 4B (agarose beads, 4%)
Particle size range: 52 – 165 µm
Molecular weight range: 6 x 104 – 2 x 107
Matrix activation: Cyanogen bromide
Matrix attachment: Amino
Ligand density: 2 – 8 μmol D-alanine / ml gel
pH stability: 4 – 9
Flow Specifications: 70 – 140 cm / h
Supplied as a suspension in 2M NaCl, 0.02% thimerosal

References:
Solid-phase enzyme-receptor assay (SPERA): a competitive-binding assay for glycopeptide antibiotics of the vancomycin class. Clin. Chem. (1985) 31: 1606-1610.
Binding of the glycopeptide antibiotic teicoplanin to D-alanyl-D-alanine-agarose: the effect of micellar aggregates. J. Appl. Biochem. (1985) 7: 133-137.
On-column derivatization of the antibiotics teicoplanin and ristocetin coupled to affinity capillary electrophoresis. Electrophoresis (2003) 24: 808-815.
Affinity partitioning of vancomycin. Biotechnol Bioeng. (1991) 38:535-44.

Related products:
L-Aspartic Acid-Separopore® 4B-CL (SKU # 20181067)
L-Glutamic Acid Separopore® 4B-CL (SKU # 20181068)
Glycine-Separopore® 4B-CL (SKU # 20181065)
L-Histidine-Separopore® 4B-CL (SKU # 20181069)
L-Lysine-Separopore® 4B-CL (SKU # 20181070)
L-Phenylalanine-Separopore® 4B-CL (SKU # 20181071)
Poly-L-Lysine-Separopore® 4B-CL (SKU # 20181075)
Glutathione-Separopore® 4B (SKU # 20181050)

Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®