D-Lactic Acid/Lactate Colorimetric Assay Kit

Detection Principle:
Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D-lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated.#N/A
Instrument: Microplate reader
Assay Time: 20 min

Tested Sample Types: Serum, plasma, animal tissue

Research Area's: Glycolysis & Carbohydrates

Shelf Life: 6 Months

Storage:
2-8℃

Citations:


Detection Principle: https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.202100253