DEAE Separopore® 6B-CL

Application: DEAE-Separopore® 6B-CL is a weak anion exchanger with excellent flow capacities and a high capacity for binding and resolution of proteins of all pI values. bio-World's DEAE-Separopore® 6B-CL was prepared by covalent attachment of diethylaminoethyl (DEAE) to Separopore® 6B-CL. DEAE remains charged and maintains high capacity over a wide working range of pH 3 – 9. It is an excellent matrix for both analytical and preparative fractionation of proteins.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical Specifications:
Ligand: Diethylaminoethyl (DEAE)
Matrix: Separopore® 6B-CL (crosslinked agarose beads, 6%)
Particle size range: 52 – 165 μm
Molecular weight range: 6 x 104 – 2 x 107
Ligand coupling method: CNBr activation
Total ionic capacity: 0.13 – 0.17 mmol (Cl-) / ml drained gel
Binding capacity: 50 – 170 mg protein / ml drained gel
Flow Specifications: 70 – 140 cm / h
pH stability: 3 – 9
Storage: 2 – 8 °C
Supplied as pre-swollen gel in 20% ethanol

References:
Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium lappa L). Chem Cent J. (2011) 5: 16.
Purification and characterization of an intracellular α-glucosidase with high transglycosylation activity from A. niger M-1. Prep Biochem Biotechnol. (2011) 41: 201-17.
Structural characterization of polysaccharides from Zizyphus jujuba and evaluation of antioxidant activity. Int J Biol Macromol. (2010) 47: 445-53.
A novel Ca2+-dependent phospholipase D from Streptomyces tendae, possessing only hydrolytic activity. Arch Pharm Res. (2009) 32: 1461-7.
A glycoprotein extracted from golden oyster mushroom Pleurotus citrinopileatus exhibiting growth inhibitory effect against U937 leukemia cells. J Agric Food Chem. (2009) 57: 6706-11.
An effective method for hemoglobin E detection: DEAE sepharose microcolumn. Lab Hematol. (2009) 15: 10-2.
Antioxidative activity of polysaccharide fractions isolated from Lycium barbarum Linnaeus. Int J Biol Macromol. (2009) 45: 146-51.
Structural characterization and anti-fatigue activity of polysaccharides from the roots of Morinda officinalis. Int J Biol Macromol. (2009) 44: 257-61.
Purification and characterization of a cathepsin L-like enzyme from the body wall of the sea cucumber Stichopus japonicus. Biosci Biotechnol Biochem. (2008) 72: 1430-7.
Purification and characterization of chitinases and chitosanases from a new species strain Pseudomonas sp. TKU015 using shrimp shells as a substrate. Carbohydr Res. (2008) 343: 1171-9.
Purification and biochemical properties of phospholipase D (PLD57) produced by Streptomyces sp. CS-57. Arch Pharm Res. (2007) 30: 1302-8.
Characterisation of secreted polysaccharides and (glyco)proteins from suspension cultures of Pyrus communis. Phytochemistry. (2008) 69: 873-81.
Purification, kinetic properties and physicochemical characterization of a novel acid phosphatase (AP) from germinating peanut (Arachis hypogaea) seed. Ital J Biochem. (2007) 56: 149-57.
Purification and characterization of an immunomodulatory Peptide from bovine placenta water-soluble extract. Prep Biochem Biotechnol. (2007) 37: 173-84.
Aspergillus niger pH 2.1 optimum acid phosphatase with high affinity for phytate. Folia Microbiol (Praha). (2006) 51: 541-5.
A novel thermostable alpha-galactosidase from the thermophilic fungus Thermomyces lanuginosus CBS 395.62/b: purification and characterization. Biochim Biophys Acta. (2007) 1770: 55-62.
Purification and characterization of cadmium-binding protein from unicelluar alga Chlorella sorokinian. Curr Microbiol. (2006) 52: 460-3.
Purification and characterization of arginine kinase from locust. Protein Pept Lett. (2006) 13: 405-10.
Structural investigation of a fucoidan containing a fucose-free core from the brown seaweed, Hizikia fusiforme. Carbohydr Res. (2006) 341: 1135-46.
Differential phosphorylation patterns of P-glycoprotein reconstituted into a proteoliposome system: insight into additional unconventional phosphorylation sites. Anticancer Res. (2005) 25: 3925-35.
Analysis of mucin composition in gastric juices of chronic rheumatic patients with upper gastrointestinal damage. Biomed Res. (2005) 26: 147-51.
Purification, potency and immunogenicity analysis of Vero cell culture-derived rabies vaccine: a comparative study of single-step column chromatography and zonal centrifuge purification. Microbes Infect. (2005) 7: 1110-6.
Purification and characterization of C-Phycocyanin from cyanobacterial species of marine and freshwater habitat. Protein Expr Purif. (2005) 40: 248-55.
Purification and characterization of a fructosyltransferase from onion bulbs and its key role in the synthesis of fructo-oligosaccharides in vivo. New Phytol. (2005) 165: 513-24.
Carbohydrases in camel (Camelus dromedarius) pancreas. Purification and characterization of glucoamylase. Comp Biochem Physiol B Biochem Mol Biol. (2005) 140: 73-80.
Purification and characterization of a novel prolyl aminopeptidase from Maitake (Grifola frondosa). Biosci Biotechnol Biochem. (2004) 68: 1395-7.
Cloning and expression of the glucose-1-phosphate thymidylyltransferase gene (gerD) from Streptomyces sp. GERI-155. Mol Cells. (2004) 17: 274-80.
Expression, localization, and correlation of N-myristoyltransferase and its inhibitor in bovine eye. Invest Ophthalmol Vis Sci. (2004) 45: 1674-9.
Human salivary aggregation in Streptococcus intermedius type g strains: relationship with IgA. FEMS Immunol Med Microbiol. (2004) 41: 101-7.
Partial purification of an enzyme hydrolyzing indole-3-acetamide from rice cells. J Plant Res. (2004) 117: 191-8.
NADH-ubiquinone oxidoreductase activity in the kinetoplasts of the plant trypanosomatid Phytomonas serpens. Parasitol Res. (2004) 92: 341-6.
Identification of paraoxonase 3 in rat liver microsomes: purification and biochemical properties. Biochem J. (2003) 376: 261-8.
Purification and properties of an extracellular beta-xylosidase from a newly isolated Fusarium proliferatum. Bioresour Technol. (2003) 90: 33-8.
Detection and characterization of the Gloeosporium gloeosporioides growth inhibitory compound iturin A from Bacillus subtilis strain KS03. FEMS Microbiol Lett. (2003) 223: 47-51.
Chromatographic purification and properties of a therapeutic human protein C concentrate. J Chromatogr B Analyt Technol Biomed Life Sci. (2003) 790: 199-207.
Purification and some properties of a novel chitosanase from Bacillus subtilis KH1. J Gen Appl Microbiol. (2000) 46: 19-27.
Purification and characterization of a chitinase from Trichoderma viride. J Gen Appl Microbiol. (2001) 47: 53-61.
Purification and characterization of a novel chitinase from Burkholderia cepacia strain KH2 isolated from the bed log of Lentinus edodes, Shiitake mushroom. J Gen Appl Microbiol. (2002) 48: 25-33.
Studies on neutral exopolysaccharides produced by the ectomycorrhiza Thelephora terrestris. FEMS Microbiol Lett. (2002) 216: 145-9.
Screening and simple purification of alanine dehydrogenase in Bacillus strains. Prep Biochem Biotechnol. (2002) 32: 277-85.

Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore™