Direct Blood PCR Kit

Direct Blood PCR Kit contains all the reagents needed for quick amplification of DNA from whole blood and other body fluids. The kit can be also applied to direct amplification of exogenous target genes, such as virus, bacteria and parasites from blood. The kit is optimized with our unique inhibitor-resistant DNA polymerases and buffer for best performance. There is the choice of an inert green dye in the mix, used for direct gel loading.The PCR product can be directly loaded onto agarose gel without addition of loading dye for electrophoresis.

GC version works for GC-rich and complex target.

Highlight

Time saving: No need for DNA extraction or pre-lysis of sample as other similar commercial kits
Convenient and flexibility: Only need adding enzyme, primers and sample in 2X mix. The enzyme is packed in a separated vial. The customers are able to titrate the amount of enzyme to find the best enzyme amount for their targets. The whole master mix including the enzyme inside is available as well (Click here)
High inhibitor-resistance: Can amplify target in PCR containing up to 40% whole blood
Versatile: Formulation works with blood, saliva, body fluids, other liquid samples
Specificity: GC version works for >65% GC content and complex target, increases specificity
Simply analysis: Directly loading products for electrophoresis after PCR

Applications

Genotyping
Mutation detection
Transgene detection
Knockout analysis
Sequencing
Pathogen detection
Forensic identification