Direct Cell PCR Kit-GC

Direct Cell PCR Kit contains all the reagents needed for quick amplification of genomic DNA from cells. This kit can be also applied to direct amplification of exogenous target genes, such as virus, bacteria and parasites from cell culture. The kit is optimized with our unique inhibitor-resistant DNA polymerases and buffer for best performance. There is the choice of an inert green dye in the mix, used for direct gel loading. The PCR product can be directly loaded onto agarose gel without addition of loading dye for electrophoresis.

GC version works for GC-rich and complex target.

Highlight

Time saving: No need for DNA extration or pre-lysis of sample as other similar commercial kits
Convenient and flexibility: Only need adding enzyme, primers and sample in 2X mix. The enzyme is packed in a separated vial. The customers are able to titrate the amount of enzyme to find the best one for their targets. The whole master mix including the enzyme inside is available as well (Click here)
High inhibitor-resistance: Resistance to inhibitors in both cells and media
Specificity: GC version works for >65% GC content target, increases specificity
Simply analysis: Directly loading products for electrophoresis

Applications

Genotyping
Colony screening
Mutation detection
Pathogen detection