Direct S/P PCR Kit

Direct S/P PCR Kit contains all the reagents needed for quick amplification of DNA gene target from serum and plasma. This kit can be applied to direct amplification of exogenous target genes, such as virus, bacteria and parasites from serum and plasma. The kit is optimized with our unique inhibitor-resistant DNA polymerase and buffer for best performance. In most case, you can direct add sample into PCR master mix without pre-lysis.

There is the choice of an inert green dye in the mix, used for direct gel loading. The PCR product can be directly loaded onto agarose gel without addition of loading dye for electrophoresis.

GC version works for GC-rich and complex target.

Highlight

Time saving: Unnecessary DNA extraction or pre-lysis of sample as other similar commercial kits
Convenient: Only need adding enzyme, primers and sample in 2X mix. The enzyme is packed in a separated vial. The customers are able to titrate the amount of enzyme to find the best one for their targets. The whole master mix including the enzyme inside is available as well (Click here)
High inhibitor-resistance: Can amplify target in PCR containing up to 40% serum or plasma
Specificity: GC version works for >65% GC content target, increases specificity
Simply analysis: Directly loading products for electrophoresis after PCR

Application

ccfDNA detection
Pathogen detection