DLAT (clone ), anti-human, mouse
€388.00
In stock
SKU
MB0031
Background:
This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.
Alternative Name:
70 kDa mitochondrial autoantigen of primary biliary cirrhosis, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, Dihydrolipoamide S Acetyltransferase, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, DLAT, DLTA, E2, E2 component of pyruvate dehydrogenase complex, M2 antigen complex 70 kDa subunit, mitochondrial, ODP2, PBC, PDC E2, PDC-E2, PDCE2, Pyruvate dehydrogenase complex component E2, Pyruvate dehydrogenase complex E2 subunit, DLTA, PDCE2, PDC-E2, Pyruvate Dehydrogenase E2
Application Dilution: WB: 1:1000, ICC: 1:300, IP: 1:50~1:200
Specificity: This antibody detects endogenous levels of Pyruvate Dehydrogenase E2 and does not cross-react with related proteins
Immunogen:
Recombinant full length Human DLAT.
MW: Predicted band size: 69KDa , Observed band size: 69KDa
Swis Prot.: P10515
Purification & Purity:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
Mouse IgG1. Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.
Alternative Name:
70 kDa mitochondrial autoantigen of primary biliary cirrhosis, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, Dihydrolipoamide S Acetyltransferase, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, DLAT, DLTA, E2, E2 component of pyruvate dehydrogenase complex, M2 antigen complex 70 kDa subunit, mitochondrial, ODP2, PBC, PDC E2, PDC-E2, PDCE2, Pyruvate dehydrogenase complex component E2, Pyruvate dehydrogenase complex E2 subunit, DLTA, PDCE2, PDC-E2, Pyruvate Dehydrogenase E2
Application Dilution: WB: 1:1000, ICC: 1:300, IP: 1:50~1:200
Specificity: This antibody detects endogenous levels of Pyruvate Dehydrogenase E2 and does not cross-react with related proteins
Immunogen:
Recombinant full length Human DLAT.
MW: Predicted band size: 69KDa , Observed band size: 69KDa
Swis Prot.: P10515
Purification & Purity:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
Mouse IgG1. Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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