DNase I

DNase I is a recombinant endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri- and oligonucleotide products (on average producing tetranucleotides) with 5’-phosphorylated and 3’-hydroxylated ends.

It acts on single-stranded DNA, double-stranded DNA, RNA-DNA hybrids and chromatin. DNase I requires bivalent cations (Mg²⁺ and Ca²⁺) for maximal activity.

GMP-grade reagent also available.

Advantages & Features
- Recombinant: bovine Pancreatic, purified from E. coli (29 kDa monomer).
- High specific activity: more than 2,000 Kunitz units per mg protein.
- High Quality: free of RNase and Proteinase contamination.
- Complete solution: supplied with 10x Reaction Buffer.

Specifications
Unit Definition:
One Kunitz unit is defined as the amount of enzyme required for the complete degradation of 1 µg of plasmid DNA in 10 minutes at 37 ˚C.
Note: One Kunitz unit equals to 50 Worthington units.

Includes
- 1 mL DNase I (10 mg/mL)
- 1 mL Reaction Buffer (10x)
* 10x Reaction Buffer: 100 mM Tris-HCl (pH 7.5 at 25°C), 25 mM MgCl2, 1 mM CaCl2

Applications
- Removal of residual genomic DNA from RNA samples.
- Degradation of DNA template in transcription reactions.
- DNAse I footprinting.
- Perform Nick Translation.

Shipping & Storage
Shipped in: Gel pack.
Storage: -20 °C.