DNase I Set, RNase-free

On-membrane DNase I Set (RNase-Free) is designed for a highly efficient on-column digestion of DNA during critical RNA purification procedures for downstream applications requiring DNA-free total RNA.

DNase I, RNase free, recombinant, is an endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri-, and oligonucleotide products (on average producing tetranucleotides) with 5′-phosphorylated and 3′-hydroxylated ends. DNAse I acts on single-stranded DNA, double-stranded DNA, RNA-DNA hybrids, and chromatin. DNase I requires bivalent cations (Mg2+ and Ca2+) for maximal activity.

Advantages & Features
- Highly Efficient on-column digestion of DNA thanks to its innovative OM Buffer composition that ensures that the RNA remains bound to the column.
- RNAse-free.
- Recombinant Bovine Pancreatic DNAse I, purified from coli (29 kDa monomer).
- Optimized Buffer included for on-column DNase digestion.

Specifications
Unit Definition:
One Kunitz unit is defined as the amount of DNase I that causes an increase in A260 of 0.001 per minute per ml at 25 °C, pH 5.0, with highly polymerized DNA as substrate.

Includes
Includes for 50 preps:
- 250 μL DNase I (2U*/ μL)
- 2.5 mL OM Buffer
*Unit Definition ((Kunitz): One Kunitz unit is defined as the amount of DNase I that causes an increase in A260 of 0.001 per minute per ml at 25 °C, pH 5.0, with highly polymerized DNA as substrate.

Applications
Removal of Genomic DNA during the extraction of Total RNA using any Spin column Purification RNA Kit.

Shipping & Storage
Shipped in: Gel pack.
Storage: DNase I at -20°C and OM Buffer at Room Temperature.