EBI-3 mouse active recombinant protein
€295.00
In stock
SKU
600178
Protein Family: Cytokines
Pathway and Disease: Immune System, Signaling Molecules and Interaction, Cell Communication, Cancers
Alternate Names: Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein homolog, EBV-induced gene 3 protein, EBI3, Il17bDescription:
Epstein-Barr Virus Induced Gene-3 (EBI-3 or IL27-beta) is a secreted glycoprotein belonging to the hematopoietin receptor family and related to the p40 subunit of IL-12. It was identified by its induced expression in B-lymphocytes in response to Epstein-Barr virus infection. EBI-3 forms heterodimers with p28 to form IL-27 and with p35 to form IL-35. Both IL-27 and IL-35 have anti-inflammatory and regulatory activity. This cytokine can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and has diverse effects on innate immune cells. It synergizes with IL-12 to trigger interferon-gamma production in naïve CD4 T-cells, and binds to the cytokine receptor WSX-1/TCCR. It also has antitumor and antiangiogenic activity by inducing the production of antiangiogenic chemokines. Recombinant Mouse EBI is a non-glycosylated polypeptide chain consisting of 207 amino acids with a MW of 22.9 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Assay data for mouse recombinant EBI-3 is based upon qualitative binding to anti-EBI-3 antibody.
Accession No.: O35228
MW: 22900 Da
Sequence:
MALVALSQPR VQCHASRYPV AVDCSWTPLQ APNSTRSTSF IATYRLGVAT QQQSQPCLQR SPQASRCTIP DVHLFSTVPY MLNVTAVHPG GASSSLLAFV AERIIKPDPP EGVRLRTAGQ RLQVLWHPPA SWPFPDIFSL KYRLRYRRRG ASHFRQVGPI EATTFTLRNS KPHAKYCIQV SAQDLTDYGK PSDWSLPGQV ESAPHKP
Purity:
Purity > 90% as determined by analytical HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug protein lyophilized in 10 mM sodium citrate pH3.0. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Immune System, Signaling Molecules and Interaction, Cell Communication, Cancers
Alternate Names: Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein homolog, EBV-induced gene 3 protein, EBI3, Il17bDescription:
Epstein-Barr Virus Induced Gene-3 (EBI-3 or IL27-beta) is a secreted glycoprotein belonging to the hematopoietin receptor family and related to the p40 subunit of IL-12. It was identified by its induced expression in B-lymphocytes in response to Epstein-Barr virus infection. EBI-3 forms heterodimers with p28 to form IL-27 and with p35 to form IL-35. Both IL-27 and IL-35 have anti-inflammatory and regulatory activity. This cytokine can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and has diverse effects on innate immune cells. It synergizes with IL-12 to trigger interferon-gamma production in naïve CD4 T-cells, and binds to the cytokine receptor WSX-1/TCCR. It also has antitumor and antiangiogenic activity by inducing the production of antiangiogenic chemokines. Recombinant Mouse EBI is a non-glycosylated polypeptide chain consisting of 207 amino acids with a MW of 22.9 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Assay data for mouse recombinant EBI-3 is based upon qualitative binding to anti-EBI-3 antibody.
Accession No.: O35228
MW: 22900 Da
Sequence:
MALVALSQPR VQCHASRYPV AVDCSWTPLQ APNSTRSTSF IATYRLGVAT QQQSQPCLQR SPQASRCTIP DVHLFSTVPY MLNVTAVHPG GASSSLLAFV AERIIKPDPP EGVRLRTAGQ RLQVLWHPPA SWPFPDIFSL KYRLRYRRRG ASHFRQVGPI EATTFTLRNS KPHAKYCIQV SAQDLTDYGK PSDWSLPGQV ESAPHKP
Purity:
Purity > 90% as determined by analytical HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug protein lyophilized in 10 mM sodium citrate pH3.0. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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