EBV (clone EBS-I-024), anti-EBV

Source:
A Boizzi mouse was immunized with EBNA1 synthetic peptide (AA 424-460).
Fusion partner: Sp2/0.

Specifications:
EBS-I-024 reacts with a conserved domain on EBNA1 (AA 430-442), present in all EBV isolates. EBNA1 is expressed in all viral latency stages in vitro and in vivo.

Species reactivity:
Positive: EBV. Negative: Other herpes viruses.

Applications:
Immunohistochemistry and IFA on cultured cells: strongly positive on EBV+ tumor tissue by immunohistochemistry on FFPE sections. Strongly positive on immunoblot. Suitable for EBNO typing. Used in antigen capture ELISA. Suitable for functional studies: not interfering with DNA binding.
ELISA: +
Immunofluorescence: +
Paraffin sections: +
Western blotting: +

Positive control: EBV transformed cells.

References:
- Chen, MR et al. J. Virol.67(8): 4875-85 (1993).
- Chen, MR et al. J. Biomed. Sci. 5(3): 173-9 (1998).
- Sivachandra, N, et al, J. Virol.86(1): 60-68 (2012)