EndoG (clone 7F2G10), anti-human, rat
€396.00
In stock
SKU
253261
Catalog Nr.: 253261
Size: 0.1 mg
Isotype: mouse IgG1
Applications: E, WB
Size: 0.1 mg
Isotype: mouse IgG1
Applications: E, WB
Protein Family: Enzymes
Pathway and Disease: Cell Growth and Death
Description:
The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.
Alternate Names: EndoG: Endonuclease G
Application Notes: E: 1:500-1:1,000; WB: 1:100-1:500
Accession No.: Q14249
Antigen: Amino acids 51 – 140 of human EndoG.
Format: Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.02% sodium azide. Antibody was purified by Protein-A affinity chromatography.
Storage:
Store at 4°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.
Pathway and Disease: Cell Growth and Death
Description:
The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.
Alternate Names: EndoG: Endonuclease G
Application Notes: E: 1:500-1:1,000; WB: 1:100-1:500
Accession No.: Q14249
Antigen: Amino acids 51 – 140 of human EndoG.
Format: Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.02% sodium azide. Antibody was purified by Protein-A affinity chromatography.
Storage:
Store at 4°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.
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