Epithelial Glycoproteins (clone MFG-06), anti-human, rat

Source:
A BALB/c mouse was immunized with a milk fat globule membrane preparation derived from human breast cancer cell line PMC42.
Fusion partner: P3-X63-Ag8.653.

Specifications:
MFG-06 reacts with a glycoprotein of 40-45 kDa, known as Milk fat globule-EGF factor 8 protein (MFG-E8), lactadherin, p47 or milk fat globule 1 antigen. It is present on normal epithelial cells in various organs and considered a differentiation marker in carcinomas. It contains one EGF-like domain and 2 F5/8 type C domains. Functioning as a specific ligand forIntegrin β5 and Integrin β3, MFG-E8 is thought to be involved in gamete interactions and cell attachment, possibly playing a role in fertilization and apoptosis. Additionally, MFG-E8 binds to rotavirus and inhibits its replication, thereby protecting the cell from viral infection. Overexpression of MFG-E8 is associated with breast cancer, suggesting that MFG-E8 may be related to tumorigenesis or progression. Among testicular carcinomas, seminomas are negative.

Species reactivity:
Positive: human, rat.

Applications:
Classification of carcinomas. Distinguish embryonal carcinomas from seminomas.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: Normal breast or breast carcinoma.

References:
- Bártková J, et. al. Tumour Biol. 8(1):45-56(1987).
- Yamazaki, M. et al. Lab. Invest.94: 1260-72 (2014).
- Alfaro-Lira, S. et al. 2012. Int J Environ Res Public Health.9: 1630-1648 (2012)