Estrogen receptor (clone AER -14), anti-human, mouse, bovine, rabbit, rat

Source:
A BALB/c mouse was immunized with estrogen receptor from calf uterus.
Fusion partner: SP2/0.

Specifications:
AER-314 reacts with ER (67 kDa), an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. Structurally ER consists of 6 functional domains (domain A-F). Functional mapping of the estrogen receptor has determined a transcriptional promoting activity in the A/B domain. The hormone-binding domain (E domain) is located towards the carboxy terminal, whereas the DNA-binding domain (C-domain) is found in the central portion of the molecule. It has been speculated that the presence in breast cancer cells of truncated forms of estrogen receptor lacking the hormone-binding domain might promote the uncontrolled growth of the tumor. AER-314 epitope is located at the amino terminal end (aa 120-170; B domain) of ER.

Species reactivity:
Positive: cow, human, mouse, rabbit, rat.

Applications:
AER-314 is suitable for formalin-fixed, paraffin-embedded tissues.
Frozen sections: +
Paraffin sections: Citrate

Positive control: Human uterus, ER positive breast cancer, MCF-7 cells.

References:
- Abbondanza C et. al. Steroids58: 4-12 (1993).