Estrogen receptor (clone ER-69), anti-human

Source:
A BALB/c mouse was immunized with recombinant human estrogen receptor alpha protein (aa2-185).
Fusion partner: SP2/0.

Specifications:
ER-69 is specific to ER alpha (67 kDa) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. Structurally ER consists of 6 functional domains (domain A-F). Functional mapping of the estrogen receptor has determined a transcriptional promoting activity in the A/B domain. The hormone-binding domain(E domain) is located towards the carboxy terminal, whereas the DNA-binding domain(C-domain) is found in the central portion of the molecule. It has been speculated that the presence in breast cancer cells of truncated forms of estrogen receptor lacking the hormone-binding domain might promote the uncontrolled growth of the tumor. The ER-69 epitope is located in the transcriptional promoting (A/B) domain of ER).

Species reactivity:
Positive: human.

Applications:
ER-69 can be used for staining of formalin-fixed, paraffin-embedded breast carcinomas.
Frozen sections: +
Paraffin sections: Citrate

Positive control: Human uterus, ER positive breast cancer, MCF-7 cells.

References:
- Zafrani, B. et al. Histopatholoy37(6): 536-45 (2000).
- Harvey J. et al. J. Clin. Oncol.17(5): 1474-81 (1999)