Exosome Detachment Solution RUO
€0.00
In stock
SKU
IS-EDStep
INTRODUCTION
Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB)1, with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases.
The biological characterization of exosomes requires in most cases the isolation of intact exosomes. In this sense, many methods have been developed for the isolation of exosomes from biological fluids, among which are ultracentrifugation, chromatography, filtration, polymer-bases precipitation and immunological separation2. As a rule, the use of immunological methods requires the use of a solid substrate, usually magnetic beads, coated with antibodies or ligands directed against specific antigens on the exosome surface, allowing their specific isolation. In this way, in some cases for downstream exosome analysis it is necessary to elute the exosomes from the antibody-bead complex, avoiding potential interference.
PRODUCT DESCRIPTION
The solution of detachment of exosomes from antibody-bead complex is based on chaotropic agent and low pH (3) that allow a high recovery without destabilizing exosomes2 by means of an easy protocol.
INTENT OF USE
This solution has been designed to elute the exosomes from the antibody-bead complex and allow downstream analysis of the immunopurified exosomes without any type of interference. This product is compatible with #ExoStep kits family and #Capture beads product range.
Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB)1, with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases.
The biological characterization of exosomes requires in most cases the isolation of intact exosomes. In this sense, many methods have been developed for the isolation of exosomes from biological fluids, among which are ultracentrifugation, chromatography, filtration, polymer-bases precipitation and immunological separation2. As a rule, the use of immunological methods requires the use of a solid substrate, usually magnetic beads, coated with antibodies or ligands directed against specific antigens on the exosome surface, allowing their specific isolation. In this way, in some cases for downstream exosome analysis it is necessary to elute the exosomes from the antibody-bead complex, avoiding potential interference.
PRODUCT DESCRIPTION
The solution of detachment of exosomes from antibody-bead complex is based on chaotropic agent and low pH (3) that allow a high recovery without destabilizing exosomes2 by means of an easy protocol.
INTENT OF USE
This solution has been designed to elute the exosomes from the antibody-bead complex and allow downstream analysis of the immunopurified exosomes without any type of interference. This product is compatible with #ExoStep kits family and #Capture beads product range.
| Is Featured? | No |
|---|
Write Your Own Review