Exosome Precipitation Solution RUO
€0.00
In stock
SKU
IS-EPStep
Exosome Precipitation Solutions
Easy and Rapid Precipitation Solutions:
Increasing Biomarker Sensitivity detection
- Ultracentrifugation free method for a highly-sensitive biomarker detection
- Very clean exosome preparations: reducing carry-over of albumins and immunoglobulins
- Obtain intact exosomes suitable for a wide variety of protein-sensitive applications
PRODUCT DESCRIPTION
Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB)1, with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases.
The biological characterization of exosomes requires in most cases the isolation of intact exosomes. In this sense, a large number of methods have been developed for the isolation of exosomes from biological fluids, among which are ultracentrifugation, chromatography, filtration, immunological separation and polymer-based precipitation2. Each one of these methods presents its advantages and disadvantages, being the duration of the method, the need to have specialized equipment, the volume of sample, the purity and the low recovery, some of the disadvantages that these methods present.
The solution of precipitation of Exosomes (EPS) is framed within the methods of precipitation of exosomes based on polymers, and among its main advantages it stands out its simplicity, speed, reproducibility, the slight effect in isolated exosomes and the use of neutral pH. And thanks to its specific formulation, it limits some of the disadvantages of this type of methods such as the co-isolation of non-vesicular contaminants, including lipoproteins and the presence of polymer material that might not be compatible with the downstream analysis.
• Tested application: BCA, WB, NTA, qPCR and Flow Cytometry
• Species reactivity: Human
• Recommended usage: Immunostep’s Exosome Precipitation Solution, is intended for the extracellular vesicles (EVs) and specifically exosomes (~50-150 nm) from cell culture media and biofluids (plasma, serum, urine).
• Presentation: liquid
Easy and Rapid Precipitation Solutions:
Increasing Biomarker Sensitivity detection
- Ultracentrifugation free method for a highly-sensitive biomarker detection
- Very clean exosome preparations: reducing carry-over of albumins and immunoglobulins
- Obtain intact exosomes suitable for a wide variety of protein-sensitive applications
PRODUCT DESCRIPTION
Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB)1, with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases.
The biological characterization of exosomes requires in most cases the isolation of intact exosomes. In this sense, a large number of methods have been developed for the isolation of exosomes from biological fluids, among which are ultracentrifugation, chromatography, filtration, immunological separation and polymer-based precipitation2. Each one of these methods presents its advantages and disadvantages, being the duration of the method, the need to have specialized equipment, the volume of sample, the purity and the low recovery, some of the disadvantages that these methods present.
The solution of precipitation of Exosomes (EPS) is framed within the methods of precipitation of exosomes based on polymers, and among its main advantages it stands out its simplicity, speed, reproducibility, the slight effect in isolated exosomes and the use of neutral pH. And thanks to its specific formulation, it limits some of the disadvantages of this type of methods such as the co-isolation of non-vesicular contaminants, including lipoproteins and the presence of polymer material that might not be compatible with the downstream analysis.
• Tested application: BCA, WB, NTA, qPCR and Flow Cytometry
• Species reactivity: Human
• Recommended usage: Immunostep’s Exosome Precipitation Solution, is intended for the extracellular vesicles (EVs) and specifically exosomes (~50-150 nm) from cell culture media and biofluids (plasma, serum, urine).
• Presentation: liquid
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