ExoStep Plasma (CD9CB+CD81PE) RUO

INTRODUCTION
Exosomes are small extracellular vesicles that are released from cells upon fusion of anintermediate endocytic compartment, the multivesicular body (MVB)1, with the plasma membrane.
They are thought to provide a means of intercellular communication (2,3) and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment (4) and the immune response (5,6).

2. PRODUCT DESCRIPTION
The kit is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD9 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.
The use of IgG2a as a control recommended. It is compatible with this kit so it can be used simultaneously. Isotypes controls are monoclonal antibodies produced against a synthetic hapten, which is normally not present in humans or animals using inf flow cytometry. They are often used as negative controls.
• Tested application: Flow Cytometry (7,8).
• Species reactivity: Human
• Storage buffer: aqueous buffered solution containing protein stabilizer and 0.09% sodium azide (NaN3).
• Recommended usage:Immunostep’s ExoStep, is intended for the immunoisolation (immunomagnetic or FACS) and Flow Cytometry analysis of pre-enriched CD63+/CD9+ human exosomes from biofluids (serum, plasma, urine) or cell culture media.
• Presentation: Liquid