FastPANGEA™ High Fidelity DNA Polymerase MasterMix

FastPANGEA™ High Fidelity DNA Polymerase is a second generation High-fidelity DNA Polymerase that offers extreme performance for all PCR applications. It generates long templates with an accuracy and speed previously unattainable with other thermostable DNA polymerases. The error rate of FastPANGEA™ DNA polymerase is at least 50-fold lower than a normal Taq DNA Polymerase.

It possesses the 5’→ 3´ DNA polymerase activity, 3´→ 5´ exonuclease activity and it generates PCR products with blunt ends. It is also suitable for amplification of long amplicons such as 10-15 kb of genomic DNA.

Advantages & Features
- Second generation High-Fidelity DNA Polymerase.
- Extreme Fidelity: error rate is more than 50 times more accurate than normal Taq DNA Polymerase.
- Robust: maximal success with minimal optimisation.
- Very Fast: extension times are 15-30 seconds/kb.
- Increased product yield: with minimal amount of enzyme.
- Versatile: ideal for routine PCR, as well as long or difficult templates.

Specifications
Unit definition:
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP’s into acid-insoluble form in 30 minutes at 75 °C under assay conditions: 25 mM TAPS-HCl, pH 9.0 (at 25 °C), 100 mM KCl, 1.5 mM MgCl2, 1 mM Beta-mercaptoethanol, 200 μM each dNTP and 10 μg activated calf thymus DNA in 50 μl.

Includes
- 4 x 1.25 mL FastPANGEA™ High Fidelity DNA Polymerase (2x)
- 500 μL DMSO (100%)
- 100 μL MgCl2 (25 mM)
*500 x 20 µl reactions
*200 x 50 µl reactions

Applications
- PCR-Cloning
- Primers extension.
- Long or difficult amplification.
- High-Throughput PCR.

Shipping & Storage
Shipped in: Gel pack.
Storage: -20 °C.