FCXM - Transplantation cross match Determination
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In stock
SKU
IS-FCXM
Anti-Human IgG (XMStep) is intended use for determine presence of anti-HLA antibodies preformed in a patient’s serum against the lymphocytes of a possible donor. It includes a mouse anti-human IgG monoclonal antibody conjugated with a flurochrome (FITC).
RECOMMENDED USAGE
Immunostep's Transplantation cross match kit intended use for determine presence of anti-HLA antibodies preformed in a patient's serum against the lymphocytes of a possible donor by flow cytometry.
CLINICAL RELEVANCE
Human leukocyte antigens (HLAs) are antigens compound of molecules found on the surface of almost every cell in an individual's tissues, and also on white blood cells. When two people share the same HLA, they are said to be a "match", that is, their tissues are immunologically compatible with each other.
The presence of recipient antibodies against antigens expressed on donor white cells is a major risk factor for early rejection or graft loss after kidney transplant.1 In this sense, is indicated the HLA crossmatch study between donor cells and recipient serum prior to transplantation.
Analysis of specific antibodies against B and T lymphocytes by flow cytometry allows clinicians to perform transplant cross-matching tests by detecting antibodies specific for HLA class I and class II as well as other antigens also expressed in these cells.2-4 Thereby, a negative crossmatch justified proceeding with the transplant, whereas a positive crossmatch was considered a contraindication for kidney transplantation.
PRINCIPLES OF THE TEST
The anti-IgG monoclonal antibody recognize anti-donor antibodies against T-lymphocytes (anti-HLA class I antibodies) and B-lymphocytes (anti-HLA class I and/or HLA class II antibodies) on the surface of donor cells.
To identify these antibodies, the cells are incubated with the serum patient and the anti-IgG. Sample is analysed by flow cytometry.
RECOMMENDED USAGE
Immunostep's Transplantation cross match kit intended use for determine presence of anti-HLA antibodies preformed in a patient's serum against the lymphocytes of a possible donor by flow cytometry.
CLINICAL RELEVANCE
Human leukocyte antigens (HLAs) are antigens compound of molecules found on the surface of almost every cell in an individual's tissues, and also on white blood cells. When two people share the same HLA, they are said to be a "match", that is, their tissues are immunologically compatible with each other.
The presence of recipient antibodies against antigens expressed on donor white cells is a major risk factor for early rejection or graft loss after kidney transplant.1 In this sense, is indicated the HLA crossmatch study between donor cells and recipient serum prior to transplantation.
Analysis of specific antibodies against B and T lymphocytes by flow cytometry allows clinicians to perform transplant cross-matching tests by detecting antibodies specific for HLA class I and class II as well as other antigens also expressed in these cells.2-4 Thereby, a negative crossmatch justified proceeding with the transplant, whereas a positive crossmatch was considered a contraindication for kidney transplantation.
PRINCIPLES OF THE TEST
The anti-IgG monoclonal antibody recognize anti-donor antibodies against T-lymphocytes (anti-HLA class I antibodies) and B-lymphocytes (anti-HLA class I and/or HLA class II antibodies) on the surface of donor cells.
To identify these antibodies, the cells are incubated with the serum patient and the anti-IgG. Sample is analysed by flow cytometry.
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