First Strand cDNA Synthesis Kit

First-Strand cDNA Synthesis Kit is an Efficient & Sensitive System for First strand cDNA Synthesis from RNA templates. It includes s all the necessary components to synthesize first-strand cDNA, except the template RNA (total RNA or mRNA).

First-Strand cDNA Synthesis Kit features two optimized mixes, RT Enzyme Mix and RT Reaction Mix. RT Enzyme Mix combines Reverse Transcriptase and RNase Inhibitor, while RT Reaction Mix contains dNTPs, oligo(dT)18 primers, random hexamers, MgCl2 and an optimized buffer. The synthesized single-stranded cDNA is suitable for real-time quantitative PCR applications. The Kit has been formulated to provide high yields of full-length cDNA product and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg to 5 μg of total RNA.

Advantages & Features
- Convenient: the Kit includes all the components needed.
- Highly Sensitive: full length cDNA starting at 10 pg – 5 μg of total RNA, due a novel RNase H treatment.
- Full-length first strand cDNA up to 10kb.
- Optimized to increase sensitivity in RT-qPCR and RT-PCR assays.
- Optimal reaction temperature: 50 °C
- Avoids RNA template degradation.

Includes
Includes for 50 rxn:
- 50 μL Reverse Transcriptase (200U/µL)
- 500 μL cDNA Synthesis Buffer (5x)
- 50 μL dNTP Mix (10 mM)
- 50 μL Hexamer Primer (200ng/µL)
- 50 μL Oligo (dT)20(50µM)
- 25 μL RNase Inhibitor (50U/µL)
- 1 mL Nuclease-free Water
- 250 µL DTT (0.1 M)

Applications
• First Strand cDNA Synthesis for RT-PCR and RT-qPCR.
• Construction of full length cDNA Libraries.
• RNA Analysis.

Shipping & Storage
Shipment: at Gel Pack.
Storage: Upon receipt of the kit, immediately store the components at -20 °C in a freezer without a defrost cycle. It is recommended to reduce freeze-thaw cycles as less as possible.