Fish IL1b (Interleukin 1 Beta) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta(IL1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Beta(IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Beta(IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Beta(IL1b) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cytokine, Tumor immunity, Infection immunity

Alternative Names:
IL1-B, IL1-Beta, IL1F2, IL-1β, Interleukin-1 Family Member 2, Catabolin

Sample Type:
serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 100 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only